Integration of Embryonic Stem Cells in Metanephric Kidney Organ Culture
نویسندگان
چکیده
منابع مشابه
Integration of embryonic stem cells in metanephric kidney organ culture.
Many stages of nephrogenesis can be studied using cultured embryonic kidneys, but there is no efficient technique available to readily knockdown or overexpress transgenes for rapid evaluation of resulting phenotypes. Embryonic stem (ES) cells have unlimited developmental potential and can be manipulated at the molecular genetic level by a variety of methods. The aim of this study was to determi...
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Renal epithelial cells derive from either cells of the metanephric mesenchyme or ureteric bud cells, but the origin of other renal cells is unclear. To test whether metanephric mesenchymal cells generate cells other than epithelial, we examined the developmental potential of a metanephric mesenchymal cell line (7.1.1 cells) and of primary cultures of metanephric mesenchymal cells. 7.1.1 Cells e...
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Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...
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Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells. Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...
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Background and purpose: Spermatogenesis is a well-organized process that is influenced by a variety of factors. Alkaline phosphatase, and Gfra1, Lin28, and Sall4 genes are among the key players in this interconnected process. This study aimed to investigate the expression levels of Gfra1, Lin28, and Sall4 genes in embryonic, spermatogonial, and embryonic stem-like (ES-like) cells in mice. Mate...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 2005
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.2004070584